5IW1
Crystal Structure of B4.2.3 T-Cell Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 31 |
| Unit cell lengths | 96.112, 96.112, 167.578 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.056 - 3.001 |
| R-factor | 0.243 |
| Rwork | 0.240 |
| R-free | 0.26750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ivx |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.608 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.131 | 0.482 |
| Number of reflections | 32680 | |
| <I/σ(I)> | 12.6 | 1.8 |
| Completeness [%] | 94.4 | 65.3 |
| Redundancy | 5.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 291 | 16% PEG 4000, 0.1M TRIS, 0.2M Magnesium Chloride |
