5IVU
Crystal Structure of Streptomyces griseoflavus Cofilin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-08-02 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54187 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.927, 65.937, 70.757 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.420 |
R-factor | 0.2281 |
Rwork | 0.225 |
R-free | 0.27908 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i2q |
RMSD bond length | 0.008 |
RMSD bond angle | 1.329 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.460 |
High resolution limit [Å] | 2.420 | 2.420 |
Rmerge | 0.071 | 0.610 |
Number of reflections | 9995 | |
<I/σ(I)> | 16.86 | 1.98 |
Completeness [%] | 96.9 | 96.9 |
Redundancy | 4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277.15 | 0.1 M Tris HCl, 25% PEG 1000 |