5IVL

CshA Helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	277
Detector technology	CCD
Collection date	2011-11-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	58.489, 96.314, 83.773
Unit cell angles	90.00, 95.21, 90.00

Refinement procedure

Resolution	23.520 - 2.300
R-factor	0.205
Rwork	0.202
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hjv
RMSD bond length	0.009
RMSD bond angle	1.143
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.520	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.061
Number of reflections	40920
<I/σ(I)>	32.84
Completeness [%]	99.7
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	0.1M Sodium acetate, pH 4.6, 1.5M Ammonium sulfate