5IVA
The LPS Transporter LptDE from Pseudomonas aeruginosa, core complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2013-11-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 148.923, 155.985, 115.654 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.089 - 2.988 |
| R-factor | 0.2502 |
| Rwork | 0.248 |
| R-free | 0.29540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q35 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.668 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.089 |
| High resolution limit [Å] | 2.988 |
| Number of reflections | 27493 |
| <I/σ(I)> | 12.4 |
| Completeness [%] | 100.0 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 100 mM sodium citrate pH 5.5, 100 mM sodium chloride, 100 mM lithium chloride, 12% PEG 4000 |
