5IV8
The LPS Transporter LptDE from Klebsiella pneumoniae, core complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2013-05-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.020, 173.050, 84.757 |
| Unit cell angles | 90.00, 111.26, 90.00 |
Refinement procedure
| Resolution | 41.414 - 2.938 |
| R-factor | 0.2378 |
| Rwork | 0.235 |
| R-free | 0.28390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q35 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.488 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.580 |
| High resolution limit [Å] | 2.938 |
| Number of reflections | 41834 |
| <I/σ(I)> | 16.6 |
| Completeness [%] | 97.4 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 22% PEG4K, 200mM ammonium phosphate |
