5IUY
Structural insights of the outer-membrane channel OprN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-08 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | I 4 |
| Unit cell lengths | 257.648, 257.648, 81.511 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 182.180 - 2.290 |
| R-factor | 0.18993 |
| Rwork | 0.188 |
| R-free | 0.22536 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d5k |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.074 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 182.185 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.149 | 0.755 |
| Number of reflections | 119764 | |
| <I/σ(I)> | 8.04 | 1.76 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 4.88 | 4.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Sodium acetate pH 5.4, 0.8 M Sodium formate, 11% PEG 4000 |
