5IU6

Crystal structure of E.coli purine nucleoside phosphorylase with 7-deazahypoxanthine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.8
Spacegroup name	P 61 2 2
Unit cell lengths	120.370, 120.370, 238.971
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.860 - 2.510
R-factor	0.15701
Rwork	0.155
R-free	0.20047
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.428
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.870	2.650
High resolution limit [Å]	2.510	2.510
Rmerge	0.065	0.170
Number of reflections	35075
<I/σ(I)>	10.4375
Completeness [%]	98.1
Redundancy	4.47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		294	AMMONIUM SULPHATE