5ITE
2.2-Angstrom in meso crystal structure of Haloquadratum Walsbyi Bacteriorhodopsin (HwBR) from Octylglucoside (OG) Detergent Micelles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.442, 61.565, 119.689 |
| Unit cell angles | 90.00, 116.80, 90.00 |
Refinement procedure
| Resolution | 47.506 - 2.182 |
| R-factor | 0.2609 |
| Rwork | 0.259 |
| R-free | 0.28930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qi1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.389 |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.510 | 2.300 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.171 | 0.602 |
| Number of reflections | 35898 | |
| <I/σ(I)> | 6 | 2.1 |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 3.3 | 3.2 |
| CC(1/2) | 0.987 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | 293 | 8% (v/v) Tacsimate, 20% (v/v) PEG 3350 |
