5ITB

Crystal structure of the anti-RSV F Fab 14N4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	80
Detector technology	CCD
Collection date	2015-09-22
Detector	Bruker Platinum 135
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	44.447, 75.129, 61.374
Unit cell angles	90.00, 93.86, 90.00

Refinement procedure

Resolution	28.353 - 2.000
R-factor	0.1947
Rwork	0.194
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q9q
RMSD bond length	0.012
RMSD bond angle	1.361
Data reduction software	XPREP
Phasing software	PHENIX ((1.10.1_2155:000))
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.360	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.118	0.496
Number of reflections	27310
<I/σ(I)>	8.8	2.2
Completeness [%]	99.6	100
Redundancy	4.4
CC(1/2)	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3350, 50 mM zinc acetate