5ISX
Structure of the holo PCP-E didomain of the gramicidin S synthetase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 224.812, 58.039, 90.857 |
| Unit cell angles | 90.00, 100.64, 90.00 |
Refinement procedure
| Resolution | 38.971 - 2.335 |
| R-factor | 0.1882 |
| Rwork | 0.187 |
| R-free | 0.22520 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.747 |
| Data reduction software | XDS (OSX10.9.5_Darwin13.4.0) |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.971 |
| High resolution limit [Å] | 2.335 |
| <I/σ(I)> | 3.38 |
| Completeness [%] | 99.3 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M MgCl2, 18% PEG 3350, 5% glycerol, and 100 mM sodium cacodylate pH 6.5 |
