5ISF

Crystal structure of mouse CARM1 in complex with inhibitor SA0705

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	74.742, 98.137, 206.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.220
R-factor	0.1674
Rwork	0.166
R-free	0.20130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
RMSD bond length	0.003
RMSD bond angle	0.736
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_1980)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	73738
<I/σ(I)>	16.7	3.5
Completeness [%]	98.7	98.3
Redundancy	4.4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	297	Tris-HCl pH 8.0 100 mM PEG 2000 MME 10 % NaCl 167 mM