5ISB

Crystal structure of mouse CARM1 in complex with inhibitor SA0435

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	P 21 21 2
Unit cell lengths	74.430, 98.082, 205.678
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.1555
Rwork	0.154
R-free	0.18990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
RMSD bond length	0.012
RMSD bond angle	1.316
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	102846
<I/σ(I)>	27.4
Completeness [%]	99.9	100
Redundancy	6.7	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	297	Tris-HCl pH 8.0 100 mM PEG 2000 MME 19% NaCl 133 mM