5IS2
Crystal structure of Mycobacterium avium SerB2 at pH 6.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.978 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 66.476, 109.494, 133.412 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.139 - 1.881 |
R-factor | 0.2048 |
Rwork | 0.203 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3p96 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.959 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.880 |
Number of reflections | 38932 |
<I/σ(I)> | 29 |
Completeness [%] | 98.4 |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 22% PEG 8000, 0.1M Magnesium acetate tetrahydrate, 0.1M HEPES pH 6.6 |