5IRI

Structure of the mouse SAD-B AIS-KA1 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97910
Spacegroup name	P 42 21 2
Unit cell lengths	86.839, 86.839, 91.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.300 - 2.800
R-factor	0.2234
Rwork	0.221
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yom
RMSD bond length	0.009
RMSD bond angle	0.981
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.114	0.655
Number of reflections	9101
<I/σ(I)>	23.84	5.36
Completeness [%]	99.9	100
Redundancy	9.9	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.51	294	0.1 M HEPES, 3M KCl, 0.01 M Urea