5IRG

Reverse transcriptase domain of group II intron maturase from Roseburia intestinalis in P212121 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	42.810, 145.350, 200.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.135 - 2.300
R-factor	0.1926
Rwork	0.191
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hhj
RMSD bond length	0.002
RMSD bond angle	0.430
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2254: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.135
High resolution limit [Å]	2.300	2.300
Number of reflections	57600
<I/σ(I)>	16.9
Completeness [%]	99.8
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291.15	100 mM MMT pH 8.0, 25% PEG 1500