5IQQ
Crystal structure of the human RBM7 RRM domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9720 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.707, 83.210, 60.826 |
| Unit cell angles | 90.00, 119.63, 90.00 |
Refinement procedure
| Resolution | 49.085 - 2.520 |
| R-factor | 0.2175 |
| Rwork | 0.216 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2J0Q and 1H2V |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.691 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10-2152) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.137 | 0.903 |
| Number of reflections | 17201 | |
| <I/σ(I)> | 10.58 | 1.5 |
| Completeness [%] | 96.8 | 97.2 |
| Redundancy | 4.54 | 4.52 |
| CC(1/2) | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 292 | 0.1 M Hepes pH 7.5, 150 mM KCl, 1.4 M tri-sodium citrate |
