5IQQ

Crystal structure of the human RBM7 RRM domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9720
Spacegroup name	C 1 2 1
Unit cell lengths	115.707, 83.210, 60.826
Unit cell angles	90.00, 119.63, 90.00

Refinement procedure

Resolution	49.085 - 2.520
R-factor	0.2175
Rwork	0.216
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2J0Q and 1H2V
RMSD bond length	0.003
RMSD bond angle	0.691
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10-2152)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.137	0.903
Number of reflections	17201
<I/σ(I)>	10.58	1.5
Completeness [%]	96.8	97.2
Redundancy	4.54	4.52
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	292	0.1 M Hepes pH 7.5, 150 mM KCl, 1.4 M tri-sodium citrate