5IQP
14-3-3 PROTEIN TAU ISOFORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.290, 79.300, 101.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.967 - 2.602 |
| R-factor | 0.1891 |
| Rwork | 0.186 |
| R-free | 0.23930 |
| Structure solution method | MIR |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.398 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.967 | 2.690 |
| High resolution limit [Å] | 2.602 | 2.602 |
| Rmerge | 0.065 | 0.295 |
| Number of reflections | 17742 | |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 24% PEG4K, 140 mM LI2SO4, 4 % of SATURATED NACL IN 80 mM MES AT pH 6.4; PROTEIN 7 mg/ml |
