5IQJ

1.9 Angstrom Crystal Structure of Protein with Unknown Function from Vibrio cholerae.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	C 2 2 21
Unit cell lengths	45.126, 149.512, 159.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.410 - 1.900
R-factor	0.18315
Rwork	0.181
R-free	0.22920
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.315
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.410	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.067	0.586
Number of reflections	42909
<I/σ(I)>	20.3	2.9
Completeness [%]	99.9	99.8
Redundancy	5.2	5.1
CC(1/2)	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	Protein: 7.0 mg/ml, 0.3M Sodium chloride, 0.01M HEPES (pH 7.5); Screen: 2M Ammonium sulfate, 0.1M HEPES (pH 7.5), 2% (w/v) PEG 400, 1% P300;