5IQG
Aminoglycoside Phosphotransferase (2'')-Ia (CTD of AAC(6')-Ie/APH(2'')-Ia) in complex with GDP, Magnesium, and Gentamicin C1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-26 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.260, 98.480, 93.020 |
| Unit cell angles | 90.00, 105.26, 90.00 |
Refinement procedure
| Resolution | 89.740 - 2.500 |
| R-factor | 0.19112 |
| Rwork | 0.189 |
| R-free | 0.22990 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5byl |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.545 |
| Data reduction software | iMOSFLM (0.1.27) |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.740 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.084 | 0.938 |
| Number of reflections | 54486 | |
| <I/σ(I)> | 9 | 1.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.2 | 4.2 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 80-120mM MgCl2, 8% glycerol, 10% PEG 3350, 100mM HEPES pH 7.5 |
