5IPX
Structure of ORF49 from KSHV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-11-16 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.54920 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.630, 142.510, 71.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.615 - 2.400 |
| R-factor | 0.2121 |
| Rwork | 0.210 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.611 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.600 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 17818 | |
| <I/σ(I)> | 43.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.15 M ammonium sulfate, 0.1 M MES, 32%(w/v) PEG 4000 |
