5IPB
Human Histidine Triad Nucleotide Binding Protein 1 (hHint1) H112N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.791, 46.498, 64.254 |
| Unit cell angles | 90.00, 94.94, 90.00 |
Refinement procedure
| Resolution | 34.824 - 1.550 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.19280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tw2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.848 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.824 | 34.824 | 1.555 |
| High resolution limit [Å] | 1.550 | 7.174 | 1.550 |
| Rmerge | 0.051 | 0.056 | 0.205 |
| Rmeas | 0.062 | 0.069 | 0.264 |
| Rpim | 0.034 | 0.040 | 0.164 |
| Total number of observations | 102047 | 894 | 664 |
| Number of reflections | 33064 | ||
| <I/σ(I)> | 14 | 21.8 | 4.7 |
| Completeness [%] | 97.8 | 93 | 91.1 |
| Redundancy | 3.1 | 2.6 | 2.3 |
| CC(1/2) | 0.996 | 0.992 | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 293 | 100 mM MES, 33% PEG 8000 |
