5IP2

Tomato spotted wilt tospovirus nucleocapsid protein-ssRNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	68.208, 94.732, 70.917
Unit cell angles	90.00, 112.60, 90.00

Refinement procedure

Resolution	38.578 - 3.300
R-factor	0.2847
Rwork	0.282
R-free	0.31660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4YBW
RMSD bond length	0.002
RMSD bond angle	0.531
Refinement software	PHENIX (dev_1426)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.580	38.578	3.500
High resolution limit [Å]	3.300	9.620	3.300
Rmerge	0.044	0.020	0.813
Rmeas	0.052	0.023	0.948
Total number of observations	46942
Number of reflections	12585	505	1985
<I/σ(I)>	16.51	51.71	1.75
Completeness [%]	99.0	94.4	97.8
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1M HEPES pH7.5, 25% (w/v) PEG 1000, 0.1M glycine