5IP0
PHA Binding Protein PhaP (Phasin)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MACSCIENCE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 131.785, 174.141, 198.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.852 - 3.000 |
| R-factor | 0.2298 |
| Rwork | 0.227 |
| R-free | 0.29470 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.204 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 43885 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.5M sodium acetate trihydrate, 0.15M cadmium sulfate hydrate, 0.1M HEPES buffer (pH 7.5) |
