5IOF
Structure of the transmembrane domain of the transporter SLC26Dg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.590, 82.490, 166.370 |
| Unit cell angles | 96.33, 92.94, 119.23 |
Refinement procedure
| Resolution | 19.964 - 4.200 |
| R-factor | 0.2925 |
| Rwork | 0.291 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5da0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.095 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.300 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.040 | 0.640 |
| Number of reflections | 26246 | |
| <I/σ(I)> | 13 | 1.8 |
| Completeness [%] | 97.4 | 97.8 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 50 mM Mg-acetate, 50 mM glycine, pH 9.3, PEG 400 |
