5IOD
Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-12-01 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.103, 60.487, 38.326 |
| Unit cell angles | 90.00, 93.22, 90.00 |
Refinement procedure
| Resolution | 38.270 - 1.600 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.755 |
| Data reduction software | XPREP |
| Phasing software | PHASER (2.6.1) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.014 | 0.115 |
| Number of reflections | 18788 | |
| <I/σ(I)> | 56.82 | 8.05 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
