5IOC
Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99D at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-08-21 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.033, 60.708, 38.550 |
| Unit cell angles | 90.00, 93.30, 90.00 |
Refinement procedure
| Resolution | 38.490 - 1.900 |
| R-factor | 0.1865 |
| Rwork | 0.184 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.954 |
| Data reduction software | XPREP |
| Phasing software | PHASER (2.6.1) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.018 | 0.111 |
| Number of reflections | 11341 | |
| <I/σ(I)> | 50.93 | 7.12 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 24% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
