5IOB

Crystal structure of beta-N-acetylglucosaminidase-like protein from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 2
Unit cell lengths	308.075, 91.355, 120.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.277 - 2.252
R-factor	0.2113
Rwork	0.209
R-free	0.25660
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.688
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.290
High resolution limit [Å]	2.250	6.100	2.250
Rmerge	0.144	0.081	0.507
Rmeas	0.159	0.089	0.557
Rpim	0.065	0.036	0.229
Total number of observations	931832
Number of reflections	158043
<I/σ(I)>	4.1
Completeness [%]	98.8	97.6	98.2
Redundancy	5.9	5.9	5.8
CC(1/2)		0.994	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	Ammonium sulfate, MEWS, PEG 5KMME