5IO9
X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF HUMAN DOUBLECORTIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-12-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.026, 37.783, 47.752 |
| Unit cell angles | 88.65, 105.78, 96.55 |
Refinement procedure
| Resolution | 26.000 - 1.300 |
| R-factor | 0.1876 |
| Rwork | 0.186 |
| R-free | 0.20990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ioi |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS (January 30 2009) |
| Data scaling software | SADABS (2008/1) |
| Phasing software | MOLREP (10.2.31) |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 39956 | |
| <I/σ(I)> | 13.3 | 1.8 |
| Completeness [%] | 85.7 | 46.3 |
| Redundancy | 2.2 | 2.1 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | crystals were either obtained out of 20mM CAPS pH 10.5, 100 mM NaCl, 5 mM TCEP or 20 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT |
