5IO3

Crystal structure of the legionella pneumophila effector protein RavZ - I422

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	293
Detector technology	CCD
Collection date	2014-01-20
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9794
Spacegroup name	I 4 2 2
Unit cell lengths	222.041, 222.041, 71.298
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.250 - 2.740
R-factor	0.2298
Rwork	0.224
R-free	0.28660
RMSD bond length	0.011
RMSD bond angle	1.340
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.250
High resolution limit [Å]	2.740
Number of reflections	23220
<I/σ(I)>	20.3
Completeness [%]	98.0
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	2.2M NaCl, 28% PEG 3350, 0.1M Imidazole pH6.5