5INO
Human Tdp2 reaction product (5'-phosphorylated DNA)-Mg2+ complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 69.663, 69.663, 120.677 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.832 - 3.205 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.26360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gz1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.783 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
| Rmerge | 0.082 | 0.043 | 0.574 |
| Total number of observations | 41889 | ||
| Number of reflections | 10786 | ||
| <I/σ(I)> | 8.5 | ||
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 3.9 | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 27% PEG600, 90 mM TRIS, 9% glycerol, 450 mM ammonium sulfate |
