5IMS
Saccharomyces cerevisiae acetohydroxyacid synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.683, 110.180, 180.005 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.176 - 1.984 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.19250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jsc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.485 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.840 | 2.300 |
| High resolution limit [Å] | 1.980 | 2.000 |
| Rmerge | 0.075 | 0.645 |
| Number of reflections | 130638 | |
| <I/σ(I)> | 11 | 2.1 |
| Completeness [%] | 99.3 | 96 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 273 | 34 mg/ml enzyme incubated with 1.4 mM ThDP, 0.5 mM FAD, 14 mM MgCl2, and 4.5 mM DTT. Crystals were obtained my mixing equal volumes (1 ul) of well solution (14 % PEG 4000, 0.25-0.3 M potassium phosphate, 0.2 M ammonium acetate.) and enzyme solution |
