5IMG
C9A mutant of C69-family cysteine dipeptidase from Lactobacillus farciminis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-06 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.944, 182.788, 158.523 |
| Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
| Resolution | 40.526 - 1.844 |
| R-factor | 0.1392 |
| Rwork | 0.138 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iau |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.095 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.526 | 1.860 |
| High resolution limit [Å] | 1.844 | 1.844 |
| Rmerge | 0.089 | 0.363 |
| Number of reflections | 449987 | |
| <I/σ(I)> | 22.65 | 5.11 |
| Completeness [%] | 96.4 | 92.2 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | 0.6 M 1,6-hexanediol, 19 mM CoCl2, 100 mM acetate |
