5IMC
Xanthomonas campestris Peroxiredoxin Q - Structure F3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97648 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.530, 51.240, 40.010 |
Unit cell angles | 90.00, 103.95, 90.00 |
Refinement procedure
Resolution | 10.550 - 1.050 |
R-factor | 0.1259 |
Rwork | 0.125 |
R-free | 0.14350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gkm |
RMSD bond length | 0.015 |
RMSD bond angle | 1.972 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2006: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.550 | |
High resolution limit [Å] | 1.050 | 1.050 |
Number of reflections | 56804 | |
<I/σ(I)> | 15.8 | |
Completeness [%] | 87.6 | |
Redundancy | 6.6 | |
CC(1/2) | 0.718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 30% PEG 4000, 0.1 M sodium acetate pH 5.5, 10 mM DTT |