5IMB
Crystal structure of peptidyl-tRNA hydrolase mutant-N14D from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-12-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.617, 72.381, 123.852 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.20299 |
| Rwork | 0.200 |
| R-free | 0.26188 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zxp |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.022 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-2000 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 16047 | |
| <I/σ(I)> | 28.26 | |
| Completeness [%] | 98.3 | |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1M Sodium citrate, 0.2M Ammonium acetate, 27.5% Polyethylene glycol 4000 |
