5ILX
Crystal structure of Ribosome inactivating protein from Momordica balsamina with Uracil at 1.70 Angstrom resolution
Replaces: 4FPZExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2012-05-22 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | H 3 |
Unit cell lengths | 130.186, 130.186, 39.976 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 65.090 - 1.700 |
R-factor | 0.16346 |
Rwork | 0.161 |
R-free | 0.21017 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4FPZ |
RMSD bond length | 0.021 |
RMSD bond angle | 2.013 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.090 | 2.000 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.440 | |
Number of reflections | 27751 | |
<I/σ(I)> | 37.2 | 2.7 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 14% PEG 6000, 0.1M Sodium Phosphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |