5ILV
Uninhibited ETV5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1271 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.371, 65.611, 52.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.487 - 1.800 |
| R-factor | 0.1886 |
| Rwork | 0.186 |
| R-free | 0.23450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.841 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.082 | 0.040 | 0.705 |
| Rmeas | 0.092 | ||
| Rpim | 0.039 | ||
| Total number of observations | 50220 | ||
| Number of reflections | 9566 | ||
| <I/σ(I)> | 12.2 | ||
| Completeness [%] | 99.2 | 98.2 | 97.3 |
| Redundancy | 5.2 | 5.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 20% PEG 3350, 200 mM (NH4)2SO4, pH 5.0 |
