5ILG

Crystal structure of photoreceptor dehydrogenase from Drosophila melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 2 21 21
Unit cell lengths	44.195, 93.477, 114.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.455 - 2.400
R-factor	0.1736
Rwork	0.171
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b2l
RMSD bond length	0.017
RMSD bond angle	1.376
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	114.670
High resolution limit [Å]	2.120
Rmerge	0.149
Number of reflections	27821
<I/σ(I)>	14.62
Completeness [%]	99.8
Redundancy	4.8
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	PEG 3350, phenol