5IL2

Crystal structure of SAH-bound METTL3-METTL14 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 41 21 2
Unit cell lengths	101.865, 101.865, 115.835
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.247 - 1.606
R-factor	0.1855
Rwork	0.185
R-free	0.19920
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.311
Data scaling software	Aimless
Phasing software	SHELXDE
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	1.606
Number of reflections	79825
<I/σ(I)>	21.3
Completeness [%]	99.9
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	291	PEG 8000, sodium citrate