5IKU
Crystal structure of the Hathewaya histolytica ColG tandem collagen-binding domain s3as3b in the presence of calcium at 1.9 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 296 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-02-06 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.520, 54.710, 92.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.030 - 1.900 |
| R-factor | 0.152 |
| Rwork | 0.150 |
| R-free | 0.19630 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.866 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.9.9.4 W9RSSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.030 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.425 |
| Number of reflections | 20021 | |
| <I/σ(I)> | 11.2 | 2.9 |
| Completeness [%] | 94.8 | 94.2 |
| Redundancy | 4.47 | 4.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 310 | 0.003 M calcium chloride, 0.1 M Bis-Tris pH 7.5, and 21-26% (w/v) PEG 3350. |
