5IKO
Crystal structure of human brain glycogen phosphorylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.980110 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 171.660, 171.660, 122.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.370 - 2.500 |
R-factor | 0.18916 |
Rwork | 0.187 |
R-free | 0.23855 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z8d |
RMSD bond length | 0.014 |
RMSD bond angle | 1.687 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.370 |
High resolution limit [Å] | 2.500 |
Number of reflections | 37317 |
<I/σ(I)> | 20.3 |
Completeness [%] | 99.2 |
Redundancy | 26 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 28 % (v/v) PEG 400, 0.2 M calcium chloride, and 0.1 M HEPES, pH 7.5 |