5IKE
Crystal structure of mutant-D97N of peptidyl-tRNA hydrolase from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-12-21 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.455, 71.628, 123.929 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.790 - 2.090 |
R-factor | 0.18645 |
Rwork | 0.183 |
R-free | 0.25431 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zxp |
RMSD bond length | 0.015 |
RMSD bond angle | 1.746 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-2000 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.090 | 2.100 |
Number of reflections | 23401 | |
<I/σ(I)> | 19.68 | |
Completeness [%] | 97.3 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 100 mM Sodium citrate, 200 mM Ammonium acetate, 20% Polyethylene glycol 4000 |