5IK8

Laminin A2LG45 I-form, G6/7 bound.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	I 21 21 21
Unit cell lengths	111.540, 126.290, 144.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.770 - 2.000
R-factor	0.1786
Rwork	0.177
R-free	0.20950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dyk
RMSD bond length	0.009
RMSD bond angle	0.664
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.770	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.088	1.640
Number of reflections	68770
<I/σ(I)>	17.3	1.7
Completeness [%]	100.0	99.9
Redundancy	13.5	13.6
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	100mM Ammonium Citrate diabasic 18% PEG3350