5IK7
Laminin A2LG45 I-form, Apo.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.920 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 112.068, 129.083, 147.054 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.100 - 2.000 |
| R-factor | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.21880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dyk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.662 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.100 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.133 | 4.260 |
| Number of reflections | 68371 | |
| <I/σ(I)> | 12.9 | 1.5 |
| Completeness [%] | 95.0 | 95.7 |
| Redundancy | 11.9 | 12.1 |
| CC(1/2) | 0.997 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 100mM Ammonium Citrate dibasic 18% PEG3350 |
