5IJT
Human Peroxiredoxin 2 Oxidized (SS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.014, 198.784, 116.523 |
| Unit cell angles | 90.00, 96.28, 90.00 |
Refinement procedure
| Resolution | 44.463 - 2.148 |
| R-factor | 0.2081 |
| Rwork | 0.207 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qmv |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.332 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 2.230 |
| High resolution limit [Å] | 2.148 | 2.148 |
| Rmerge | 0.066 | 0.737 |
| Number of reflections | 122120 | |
| <I/σ(I)> | 19.1 | 2.8 |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 6.1% PEG 3350, 0.1 M sodium acetate pH 4.1, 0.2 M zinc acetate |
