5IJR
X-ray structure of neuropilin-1 b1 domain complexed with Arg-1 ligand.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.139, 88.936, 40.962 |
| Unit cell angles | 90.00, 96.73, 90.00 |
Refinement procedure
| Resolution | 40.680 - 1.520 |
| R-factor | 0.18264 |
| Rwork | 0.181 |
| R-free | 0.21298 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.883 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.680 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.048 | 0.510 |
| Number of reflections | 41807 | |
| <I/σ(I)> | 22.8 | 2.8 |
| Completeness [%] | 95.4 | 67.8 |
| Redundancy | 6.6 | 5.1 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | 28% w/v PEG3350, 0.2 M AmCl |
