5IJM
Crystal Structure of an Acyl-CoA Binding Protein (LmjF.17.0620) of Leishmania major
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-10-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.989, 29.076, 33.778 |
| Unit cell angles | 103.81, 110.42, 95.00 |
Refinement procedure
| Resolution | 30.310 - 1.460 |
| R-factor | 0.16076 |
| Rwork | 0.160 |
| R-free | 0.17699 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fp5 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.175 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.310 | 1.510 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.022 | 0.075 |
| Number of reflections | 14140 | |
| <I/σ(I)> | 17.62 | 15.98 |
| Completeness [%] | 88.3 | 73.6 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 291 | 0.1M Sodium Citrate tribasic dihydrate, 10%(v/v) 2-Propanol, 20%(w/v) PEG4000 |
