5IJA

[NiFe] hydrogenase maturation protease HybD from Thermococcus kodakarensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	95
Detector technology	PIXEL
Collection date	2014-10-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	43.209, 64.467, 106.906
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.150 - 1.820
R-factor	0.2185
Rwork	0.216
R-free	0.26390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cfz
RMSD bond length	0.008
RMSD bond angle	1.020
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.820	1.820
Rmerge	0.102
Number of reflections	27433
<I/σ(I)>	13.7
Completeness [%]	98.2
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	PEG 6000, Bis-Tris propane