5IJ2

SrpA adhesin in complex with sialyllactosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	93
Detector technology	CCD
Collection date	2015-12-18
Detector	MARRESEARCH
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	174.522, 46.769, 64.761
Unit cell angles	90.00, 102.72, 90.00

Refinement procedure

Resolution	42.559 - 1.683
R-factor	0.1828
Rwork	0.182
R-free	0.20650
RMSD bond length	0.023
RMSD bond angle	1.405
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.559
High resolution limit [Å]	1.683
<I/σ(I)>	33.3
Completeness [%]	97.8
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	calcium acetate, sodium cacodylated, PEG 8000