5IIU
Crystal structure of Equine Serum Albumin in the presence of 10 mM zinc at pH 6.9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 |
Unit cell lengths | 93.746, 93.746, 141.789 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 80.010 - 2.300 |
R-factor | 0.1817 |
Rwork | 0.179 |
R-free | 0.23950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iih |
RMSD bond length | 0.008 |
RMSD bond angle | 1.247 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 80.010 | 80.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.078 | 0.028 | |
Number of reflections | 31099 | ||
<I/σ(I)> | 7.6 | ||
Completeness [%] | 99.3 | 98.4 | 99 |
Redundancy | 9.7 | 9.3 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 289 | 1 ul of 30 mg/ml protein in 10 mM Tris pH 7.5 and 150 mM NaCl buffer was mixed with 1 ul of the well condition (0.2 M Li2SO4, 0.1 M Tris:HCl, 2.0 M (NH4)2SO4, 5 mM ZnCl2, final pH 6.9) and equilibrated against well solution in 15 Well Crystallization Plate (Qiagen). Crystals were soaked with 50 mM ZnCl2 in 100 mM Tris, final pH 6.9, to final concentration of 10 mM |