5IIQ
Structure of the SPX-TTM domain fragment of the yeast inorganic polyphophate polymerase Vtc4 (form B).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000020 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 92.041, 92.041, 301.732 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.880 - 3.030 |
| R-factor | 0.2266 |
| Rwork | 0.225 |
| R-free | 0.26240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g3r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Number of reflections | 15492 | |
| <I/σ(I)> | 28.4 | 2.99 |
| Completeness [%] | 99.0 | 97.4 |
| Redundancy | 20.7 | 18.3 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES, 1.5M AmSO4, 4% PEG 1000 |
